![]() ![]() ![]() JMP 13 Windows Installers Contain Both 32-bit and 64-bit Files Learn about key features in JMP 13, watch demos and view screenshots on the JMP home page.Statistician or student, novice or expert, you’ll find something that will help you make one discovery after another. Be sure to take special note of the Registration link, as you’ll need to register to activate your free year of Technical Support.Īnd when you’re ready to start playing around with JMP, you’ll want to check out some of the links below. You should find everything you need to get JMP on your desktop. There are links to the right to ensure that your download goes smoothly. H–C polarization transfer profiles are also compared between statistically deuterated and H/D exchanged GB1.Whether you’re an administrator for a multi-license site or are installing your own personal copy of JMP for the first time, welcome! The latter O–D quadrupolar order parameter of 0.22 is significantly smaller than previously reported C–D dipolar order parameters of 0.46–0.55 for pectins, suggesting that additional motions exist at the C–O bonds in the wall polysaccharides. Application of the H–C correlation experiment to H/D exchanged Arabidopsis primary cell walls show that the O–D quadrupolar spectra of the highest polysaccharide peaks can be fit to a two-component model, in which 74% of the spectral intensity, assigned to cellulose, has a near-rigid-limit coupling, while 26% of the intensity, assigned to matrix polysaccharides, has a weakened coupling of 50 kHz. In comparison, the interior chains in the microfibril core are fully immobilized. To demonstrate the utility of this H–C technique for studying molecular motion, we show H–C correlation spectra of perdeuterated bacterial cellulose, whose surface glucan chains exhibit a motionally averaged C6 2H quadrupolar coupling that indicates fast trans-gauche isomerization about the C5–C6 bond. Our results show that multi-bond, broadband H–C polarization transfer can be achieved using 2H radiofrequency fields of ~50 kHz, relatively short contact times of 1.3–1.7 ms, and with sufficiently high sensitivity to enable D H–C correlation experiments with undistorted H spectra in the indirect dimension. In this work, we investigate the H–C magnetization transfer characteristics of one-bond perdeuterated CD spin systems and two-bond H/D exchanged C–(O)–D and C–(N)–D spin systems in carbohydrates and proteins. Recently, a Rotor Echo Short Pulse IRrAdiaTION mediated cross polarization (RESPIRATION) technique was developed, which allowed efficient transfer of H magnetization to C at moderate H radiofrequency field strengths available on most commercial MAS probes. Site-specific determination of molecular motion and water accessibility by indirect detection of H NMR spectra has advantages over dipolar-coupling based techniques due to the large quadrupolar couplings and the ensuing high angular resolution. ![]()
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